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Volume 43, Nº 2 - dezembro 2022

 

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  • Abstract / Resumo
  • References / Bibliografia
  • Citations / Citações

Revista Recursos Hídricos

DOI:10.5894/rh43n2-cti4
Este artigo é parte integrante da Revista Recursos Hídricos, Vol. 43, Nº 2, 53-62, dezembro de 2022.

Detecção de bactérias na água tratada e armazenada em reservatórios domésticos utilizando citometria de fluxo

Detection of bacteria in treated water stored in domestic reservoirs using flow cytometry

Leandro Manoel Afonso Mendes1, Hugo Sarmento2 *

* Autor correspondente: hsarmento@ufscar.br
1 Departamento de Medicina (DMed), Universidade Federal de São Carlos (UFSCar) - Rod. Washington Luiz 13565-905 - São Carlos, SP Brasil
2 Prof. Dr. - Laboratório de Biodiversidade e Processos Microbianos (LMPB), Departamento de Hidrobiologia (DHb), Universidade Federal de São Carlos (UFSCar) - Rod. Washington Luiz 13565-905 - São Carlos, SP Brasil

RESUMO

A qualidade da água é o fator individual mais importante para garantir a saúde pública. O objetivo das estações de tratamento de água é fornecer um suprimento seguro de água potável para a população, garantindo a ausência e limitando o crescimento de qualquer microrganismo que possa estar associado a patogenicidade. Independente do meio de desinfecção, é comum as bactérias regredirem durante o tratamento e distribuição da água. As bactérias são autóctones na água potável e em sua grande maioria, não podem ser cultivadas em laboratório. No entanto, as contagens baseadas em placas de cultivo são o método mais usado mundialmente como um parâmetro geral de qualidade microbiana de água potável. Diversos estudos demonstraram a presença de microrganismos na água potável ou em biofilmes utilizando técnicas mais sensíveis como a citometria de fluxo, que ainda não é utilizada no Brasil com esta aplicação. Este método é utilizado para enumeração direta das concentrações totais de células na água, utilizando marcadores de ácidos nucléicos fluorescentes e detecção de características específicas de cada célula (single-cell). Existem na literatura relatos de detecção de microrganismos, alguns patógenos, em água de distribuição, reforçando a importância de tais achados para a saúde coletiva. Além disso, os estudos existentes concentram-se em países onde não existem caixas d’água residenciais no circuito de distribuição, um elemento que pode deteriorar a qualidade da água nele armazenada. O objetivo geral deste estudo foi verificar e quantificar a presença de bactérias em caixas d’água residenciais, através da técnica de citometria de fluxo.

Palavras-chave: Rede de água tratada, água potável armazenada, parâmetros microbiológicos da água, água potável, bactérias.

ABSTRACT

Water quality is the most important factor that ensures public health. The purpose of water treatment plants is to provide a safe supply of drinking water to the population, ensuring the absence and limiting the growth of microorganisms that may be associated to pathogenicity. Regardless of the means of disinfection, it is common for bacteria to regress during the water treatment and distribution. Most drinking water bacteria cannot be grown in cultures. However, counts based on culture plates are used worldwide as a general parameter of microbiological quality of drinking water. Several studies have demonstrated the presence of microorganisms in drinking water or biofilms using more refined techniques such as flow cytometry, which is still not commonly used in Brazil with this application. This method is used for direct enumeration of total cell concentrations in water, using fluorescent nucleic acid markers and detecting specific characteristics of each cell (single-cell). There are reports in the literature of microorganisms detection, some pathogens, in distribution water, reinforcing the importance of such findings for public health. In addition, the existing studies focus on countries where there are no residential water tanks in the distribution circuit, an element that can deteriorate the quality of the water stored there. The general objective of this study was to verify and quantify the presence of bacteria in residential water tanks, using the flow cytometry technique.

Keywords: Drinking water network, stored drinking water, water microbiological parameters, drinking water, bacteria.

 

ALLEN, Martin J.; EDBERG, Stephen C.; REASONER, Donald J. - Heterotrophic plate count bacteria - What is their significance in drinking water? International Journal of Food Microbiology. ISSN 01681605. 92:3 (2004) 265–274. doi: 10.1016/ j.ijfoodmicro.2003.08.017.

BERRY, David; XI, Chuanwu; RASKIN, Lutgarde - Microbial ecology of drinking water distribution systems. Current Opinion in Biotechnology. ISSN 09581669. 2006). doi: 10.1016/j.copbio.2006.05.007.

BESMER, Michael D. et al. - The feasibility of automated online flow cytometry for in-situ monitoring of microbial dynamics in aquatic ecosystems. Frontiers in Microbiology. ISSN 1664-302X. 5:2014) 265. doi: 10.3389/fmicb.2014.00265.

BESMER, Michael D.; HAMMES, Frederik - Short-term microbial dynamics in a drinking water plant treating groundwater with occasional high microbial loads. Water Research. ISSN 0043-1354. (2016) 107:11–18. doi: 10.1016/J.WATRES.2016.10.041.

CAMPOS, Juliana Alvares Duarte Bonini et al. - Qualidade da água armazenada em reservatórios domiciliares: parâmetros físico-químicos e microbiológicos. Alimentos e Nutrição Araraquara. 14:1 (2009).

CLARK, Gemma G. et al. - Influence of point-of-use filters and stagnation on water quality at a preschool and under laboratory conditions. Water Research. ISSN 0043-1354. (2022) 211:118034.

doi: 10.1016/J.WATRES.2021.118034.

DEL GIORGIO, Paul A. et al. - Flow cytometric determination of bacterial abundance in lake plankton with the green nucleic acid stain SYTO 13. Limnology and Oceanography. ISSN 00243590. 41:4 (1996) 783–789. doi: 10.4319/lo.1996.41.4.0783.

DOUTERELO, Isabel et al. - Microbial analysis of in situ biofilm formation in drinking water distribution systems: implications for monitoring and control of drinking water quality. Applied Microbiology and Biotechnology. 100:7 (2016) 3301–3311.

EMTIAZI, Farahnaz et al. - Investigation of natural biofilms formed during the production of drinking water from surface water embankment filtration. Water Research. ISSN 00431354. 38:5 (2004) 1197–1206. doi: 10.1016/j.watres.2003.10.056.

FALKINHAM, Joseph O. et al. - Epidemiology and ecology of opportunistic premise plumbing pathogens: Legionella pneumophila, Mycobacterium avium, and Pseudomonas aeruginosa. Environmental Health Perspectives.

ISSN 15529924. 123:8 (2015) 749–758. doi: 10.1289/ehp.1408692.

FALKINHAM, Joseph O.; NORTON, Cheryl D.; LECHEVALLIER, Mark W. - Factors Influencing Numbers of Mycobacterium avium, Mycobacterium intracellulare, and Other Mycobacteria in Drinking Water Distribution Systems. Applied and Environmental Microbiology. ISSN 00992240. 67:3 (2001) 1225–1231. doi: 10.1128/AEM.67.3.1225-1231.2001.

GASOL, Josep M.; DEL GIORGIO, Paul A. - Using flow cytometry for counting natural planktonic bacteria and understanding the structure of planktonic bacterial communities. Em Scientia Marina. [S.l.] : CSIC Consejo Superior de Investigaciones Cientificas 2, 2000 GASOL, Josep M.; MORÁN, Xosé Anxelu G. - Flow Cytometric Determination of Microbial Abundances and Its Use to Obtain Indices of Community Structure and Relative Activity. Em . p. 159–187.

HAMMES, Frederik et al. - Flow-cytometric total bacterial cell counts as a descriptive microbiological parameter for drinking water treatment processes. Water Research. ISSN 0043-1354. 42:1–2 (2008) 269–277. doi: 10.1016/J.WATRES.2007.07.009.

HAMMES, Frederik et al. - Measurement and interpretation of microbial adenosine tri-phosphate (ATP) in aquatic environments. Water Research. ISSN 0043-1354. 44:13 (2010) 3915–3923. doi: 10.1016/J.WATRES.2010.04.015.

HAMMES, Frederik; EGLI, Thomas - Cytometric methods for measuring bacteria in water: Advantages, pitfalls and applications. Analytical and Bioanalytical Chemistry. ISSN 16182642. 397:3 (2010) 1083–1095. doi: 10.1007/s00216-010-3646-3.

HASSARD, Francis et al. - Understanding the Use of Flow Cytometry for Monitoring of Drinking Water. DWI Reports. 2019).

HOEFEL, Daniel et al. - Enumeration of water-borne bacteria using viability assays and flow cytometry: A comparison to culture-based techniques. Journal of Microbiological Methods. ISSN 01677012. 55:3 (2003) 585–597. doi: 10.1016/S0167-7012(03)00201-X.

JENSEN, Peter Kjær et al. - Domestic transmission routes of pathogens: the problem of in-house contamination of drinking water during storage in developing countries. Tropical Medicine & International Health. ISSN 1360-2276. 7:7 (2002) 604–609. doi: 10.1046/j.1365-3156.2002.00901.x.

KAHLISCH, L. et al. - Molecular analysis of the bacterial drinking water community with respect to live/dead status. Water Science and Technology. ISSN 02731223. 61:1 (2010) 9–14. doi: 10.2166/wst.2010.773.

KOCH, Christin et al. - Cytometric fingerprints: evaluation of new tools for analyzing microbial community dynamics. Frontiers in Microbiology. ISSN 1664-302X. 5:2014). doi: 10.3389/fmicb.2014.00273.

LEBARON, Philiipe et al. - Variations of bacterial-specific activity with cell size and nucleic acid content assessed by flow cytometry. Aquatic Microbial Ecology. ISSN 0948-3055. 28:2002) 131–140. doi: 10.3354/ame028131.

LECLERC, Henri; MOREAU, Annick - Microbiological safety of natural mineral water. FEMS Microbiology Reviews. ISSN 1574-6976. 26:2 (2002) 207–222. doi: 10.1111/j.1574-6976.2002.tb00611.x.

LIMA, F. R. A. - Reservatório domiciliar: aspecto de sua influência na qualidade da água. [S.l.] : Universidade de Sao Paulo, 1978

LIN, Wenfang et al. - Diversity and dynamics of microbial communities at each step of treatment plant for potable water generation. Water Research. (2014) 52:218–230.

MADIGAN, Michael et al. - Microbiologia de Brock. 14. ed. Porto Alegre : [s.n.]. ISBN 978-85-8271-297-9.

MOMBA, M. N. B. et al. - Overview of biofilm formation in distribution systems and its impact on the deterioration of water quality. 2000).

NEVEL, S. VAN et al. - Flow cytometric bacterial cell counts challenge conventional heterotrophic plate counts for routine microbiological drinking water monitoring. Water Research. ISSN 0043-1354. 113:2017) 191–206. doi: 10.1016/J.WATRES.2017.01.065.

NICHOLS, R. A. B.; CAMPBELL, B. M.; SMITH, H. V. - Identification of Cryptosporidium spp. oocysts in United Kingdom noncarbonated natural mineral waters and drinking waters by using a modified nested PCR-restriction fragment length polymorphism assay. Applied and Environmental Microbiology. ISSN 00992240. 69:7 (2003) 4183–4189. doi: 10.1128/AEM.69.7.4183-4189.2003.

PARK, S. R.; MACKAY, W. G.; REID, D. C. - Helicobacter sp. recovered from drinking water biofilm sampled from a water distribution system. Water Research. ISSN 00431354. 35:6 (2001) 1624–1626.

doi: 10.1016/S0043-1354(00)00582-0.

PINTO, Ameet J.; XI, Chuanwu; RASKIN, Lutgarde - Bacterial Community Structure in the Drinking Water Microbiome Is Governed by Filtration Processes. Environmental Science & Technology. ISSN 0013-936X. 46:16 (2012) 8851–8859. doi: 10.1021/es302042t.

PREST, E. I. et al. - Monitoring microbiological changes in drinking water systems using a fast and reproducible flow cytometric method. Water Research. ISSN 00431354. 47:19 (2013) 7131–7142. doi: 10.1016/j.watres.2013.07.051.

PREST, E. I. et al. - A systematic approach for the assessment of bacterial growth-controlling factors linked to biological stability of drinking water in distribution systems. Water Science and Technology: Water Supply. ISSN 16069749. 16:4 (2016) 865–880. doi: 10.2166/WS.2016.001.

RAMÍREZ-CASTILLO, Flor et al. - Waterborne Pathogens: Detection Methods and Challenges. Pathogens. ISSN 2076-0817. 4:2 (2015) 307–334. doi: 10.3390/pathogens4020307.

Ramírez-Castillo FY, Loera-Muro A, Jacques M, Garneau P, Avelar-González FJ, Harel J, Guerrero-Barrera AL. Waterborne pathogens: detection methods and challenges. Pathogens. 2015 May 21;4(2):307-34. doi: 10.3390/pathogens4020307. PMID: 26011827; PMCID: PMC4493476.

RINTA-KANTO, Johanna M. et al. - Rapid enumeration of virus-like particles in drinking water samples using SYBR green I-staining. Water Research. ISSN 00431354. 38:10 (2004) 2614–2618. doi: 10.1016/j.watres.2004.03.008.

SAFFORD, Hannah R.; BISCHEL, Heather N. - Flow cytometry applications in water treatment, distribution, and reuse: A review. Water Research. 151:2019) 110–133. ISSN 18792448.

SLOAN, William T. et al. - Quantifying the roles of immigration and chance in shaping prokaryote community structure. Environmental microbiology. 8:4 (2006) 732–740.

THOM, Claire et al. - Microbiomes in drinking water treatment and distribution: a meta-analysis from source to tap. Water Research. 2022) 118106.

ZEDER, M.; PERNTHALER, J. - Multispot live-image autofocusing for high-throughput microscopy of fluorescently stained bacteria. Cytometry Part A. 75:9 (2009) 781–788.